Billah M M, Rashid MO, Ahmed M, Yamazaki M. Antimicrobial peptide magainin 2-induced rupture of single giant unilamellar vesicles comprising E. coli polar lipids. Biochim. Biophys. Acta Biomembr. 2022 Dec 22:184112. doi: 10.1016/j.bbamem.2022.184112. Epub ahead of print. PMID: 36567034.
Highlights
• Magainin 2 (Mag) induced rupture of E. coli-lipid GUVs, inducing rapid leakage.
• Rate constant of Mag-induced rupture of GUVs (k_r) increased with Mag concentration.
• First a small micropore was formed, and grew rapidly with time to rupture the GUV.
• Binding of Mag to the outer monolayer increased the area of the GUV bilayer.
• Mechanical property of E. coli-lipid GUVs related to pore formation was elucidated.
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